Related Experiment Video
Updated: Aug 24, 2026

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry
Published on: November 20, 2014
Analysis of sugar epimers using mass spectrometry: N-acetyllactosamine-6,6'-disulfate and the 2'-epimer
Yoko Ohashi1, Yoshiyuki Itoh, Masayuki Kubota
1Center for Instrumental Analysis, The University of Electro-Communications, 1-5-1 Chofugaoka, Chofu, Tokyo 182-8585, Japan.
Abstract:
Galbeta1-4GlcNAc-6,6'-disulfate and 2'-epimer corresponding to Galbeta1- 4ManNAc-6,6'-disulfate were distinguished by mass spectrometry by utilizing fast atom bombardment (FAB), electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) methods. As for the steric information, negative-ion ESI mass spectrometry/mass spectrometry (MS/MS) provides the most extensive data, but FAB MS/MS also reveals detailed structural information of interest in our case, where MALDI MS is not yet fully equipped with post-source decay.
More Related Videos
Related Concept Videos
Mass Spectrometry: Amine Fragmentation
In amines, the number of nitrogen atoms affects the mass of the molecular ion, which is described by the nitrogen rule of mass spectrometry. This rule states that a compound containing a single or...
NMR Spectroscopy and Mass Spectrometry of Aldehydes and Ketones
Mass Spectrometry of Amines
Mass Spectrometry: Carboxylic Acid, Ester, and Amide Fragmentation
For example, the fragmentation of...
Mass Spectrometry: Aldehyde and Ketone Fragmentation

