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Detection of CD40 Protein-Umbelliferone Interaction via Differential Scanning Fluorescence
Published on: March 1, 2024
Enhanced detection sensitivity using a novel solid-phase incorporated affinity fluorescent protein biosensor
Joan Q Zhong1, Yelena Freyzon, Daniel J Ehrlich
1Whitehead Institute for Biomedical Research, MIT, Nine Cambridge Center, Cambridge, MA 02142, USA.
Biomolecular Engineering
|April 29, 2004
Abstract:
We engineered green fluorescent protein (GFP) into affinity fluorescent proteins (aFPs) biosensors. The aFPs detect protein-protein interactions by enhanced fluorescence intensity. In a proof of principle demonstration, aFPs containing haemagglutinin (HA) tag bind specifically to the anti-HA antibody. The sensitivity and specificity is enhanced 28-fold by incorporation of aFPs into solid-phase surface.
