Amperometric detection of glucose using self-catalytic carbon paste electrodes

Area of Science:

• Electrochemistry
• Biosensors
• Analytical Chemistry

Background:

• Accurate d-glucose detection is crucial for clinical diagnostics and metabolic monitoring.
• Traditional glucose sensors often rely on complex enzyme immobilization or external mediators.
• Developing robust and cost-effective glucose sensors remains an active area of research.

Purpose of the Study:

• To develop and characterize novel self-catalytic carbon paste electrodes for the electrochemical detection of d-glucose.
• To investigate the role of n-butylferrocene as both a binder and electrochemical mediator in the sensor's performance.
• To evaluate the sensor's applicability in determining d-glucose concentrations in biological samples.

Main Methods:

• Fabrication of carbon paste electrodes modified with n-butylferrocene.
• Electrochemical characterization of the modified electrodes.
• Enzymatic oxidation of d-glucose mediated by n-butylferrocene and glucose oxidase.
• Testing in model glucose solutions and spiked blood samples.

Main Results:

• The self-catalytic electrodes exhibited a linear response to d-glucose in the concentration range of 2-20 mM.
• N-butylferrocene effectively acted as a binder and mediator, simplifying electrode construction.
• A detection limit of 0.8 mM for d-glucose was achieved in a spiked blood sample.

Conclusions:

• Self-catalytic carbon paste electrodes modified with n-butylferrocene provide a promising platform for sensitive d-glucose detection.
• The integrated role of n-butylferrocene simplifies sensor design and enhances performance.
• The developed sensor demonstrates potential for practical application in blood glucose monitoring.