High-performance thin-layer chromatography with a derivatization procedure, a suitable method for the identification
Jérôme Bouligand1, Angelo Paci, Lionel Mercier
1Department of Clinical Pharmacy, Institut Gustave Roussy, 39 Rue Camille Desmoulins, 94800 Villejuif, France.
Abstract:
Busulfan is an alkylating agent widely used in combination chemotherapy regimens followed by allogeneic or autologous hematopoietic stem cell transplantation (HSCT). We present a rapid method for assaying busulfan in pharmaceutical preparations using high-performance thin-layer chromatography (HPTLC) and derivatization with 4-nitrobenzylpyridine. The method is accurate and precise and allows quantitation of busulfan in aqueous solutions from 100 to 500 microg/ml. It is suitable for identification. quantitation and stability studies of busulfan in pharmaceutical products, i.e. capsules or infusion bags prepared in a hospital pharmacy.
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