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Updated: Aug 24, 2026
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Published on: March 24, 2017
[Preparation and identification of phage-displayed ICAM-1-mimic peptide]
Wen-bo Hao1, Wei-wen Xu, Bai-hong Chen
1Institute of Tropical Medicine, First Military Medical University, Guangzhou 510515, China. haowenbo@hotmail.com
Aim:
To obtain bioactive ICAM-1 mimetic peptide.
Methods:
Phages displaying P1 and P2 were prepared by phage amplication and PEG precipitation. The binding between phage-displayed peptides and anti-ICAM-1 mAb 15.2 was evaluated by sandwich ELISA and competitive ELISA. Bioactivities of P1 and P2 was detected by immunohistochemical staining.
Results:
Phage-displayed peptides P1 and P2 could specifically bind to mAb 15.2, and the binding could be competitively inhibited by ICAM-1. Immunohistochemical staining showed that P1 and P2 could mimic the binding of ICAM-1 to its receptor LFA-1.
Conclusion:
Phage-displayed peptides P1 and P2 are bioactive just as native ICAM-1.
