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Updated: Aug 24, 2026
Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
Published on: February 6, 2018
[Preparation and identification of monoclonal antibodies against CD100 molecule]
Xiao-guang Xu1, Yong Zhu, Xue-song Liu
1Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.
Aim:
To prepare and identify the monoclonal antibodies (mAbs) against CD100.
Methods:
BALB/c mice were immunized with eukaryotic expressed CD100 protein. The splenocytes from immunized mice were fused with Sp2/0 cells and the positive hybridoma clones were screened by indirect ELISA and mAbs reacting to CD100 antigen were identified by indirect immunofluorescent staining and flow cytometry with human acute T lymphoblastic leukemia cell line MOLT-4, activated human PBMC, gibbon T lymphocyte cell line MLA-144 and monkey EBV-transformed B lymphoblast cell line(BLCL).
Results:
Seven hybridomas secreting mAbs against CD100 molecule were obtained. All the mAbs could react to natural CD100 expressed on human MOLT-4 and activated PBMCs as well as monkey BLCL cells, and six out of them could react to gibbon MLA-144 cells.
Conclusion:
A set of mAbs recognizing CD100 molecule expressed on human, monkey and gibbon cells are obtained, which may provide an useful tool for studying the structure-function relationship of CD100 molecule of human, as well as gibbon and monkey.
