Related Experiment Video
Updated: Aug 24, 2026
Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis
Published on: September 7, 2022
[Induction of IgG and IgM production by Epstein-Barr virus protein]
Yan-guo Zhang1, Meng Fu, Yu-fengg Liu
1Center of Dermatology of Chinese PLA, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Aim:
To detect the production of IgG and IgM by cultured umbilical blood B cell stimulated by UV and heat-inactivated Epstein-Barr virus (EBV).
Methods:
Mononuclear cells were isolated routinely from umbilical blood, then monocytes, NK cells and cytotoxic T cells were eliminated by L-leucine methy ester method; remained T cells were eliminated by AET-SRBC rosette method. The purified B cells were treated with UV or heat-inactivated EBV respectively and cultured in complete IMDM. The IgG and IgM in the supernatant were detected by ELISA.
Results:
The IgG and IgM production(A value) in UV-inactivated EBV group increased from 18th day to 30 th day after stimulation compared with the baseline. The A value of all time points in heat-inactivated EBV group had no significant difference.
Conclusion:
UV-inactivated EBV may induce IgG and IgM production with a time-effect relationship, suggesting that EBV protein is a key component of stimulating Ig production. This result provide the basis for the further researching the effects of functional EBV protein on the production of natural autoantibodies (NAA).
Related Concept Videos
01:29Immune Response Against Viral Pathogens
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
01:10Antibody Structure
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
01:23Immunological Memory
What is Immunological Memory?
Immunological memory is an integral function of the immune system that allows it to recognize and react more rapidly and effectively to pathogens previously encountered. This feature is...
01:15Transcytosis of IgG
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
01:22Development of Immunocompetence
The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...