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Updated: Aug 24, 2026

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient
Published on: September 17, 2011
Presence of translation elongation factor-1A in the rat cerebellar postsynaptic density
Il Soo Moon1, Sun-Jung Cho, Jae Seob Jung
1Department of Anatomy, College of Medicine, Dongguk University, 707 Sukjang, Gyeongju 780-714, South Korea. moonis@dongguk.ac.kr
Abstract:
This study examined a 55 kDa protein in the rat cerebellar postsynaptic density (PSD) fraction. The amino acid sequence of an HPLC-purified peptide derived from tryptic digestion of the protein was contained in eukaryotic translation elongation factor-1A (eEF1A, formerly known as eEF-1alpha). Immunoblot analysis showed that eEF1A is enriched in the PSD fraction and is tightly associated with the PSD 'core'. The association of eEF1A with the PSD was further evidenced by colocalization of the protein with PSD95, a PSD marker, in dissociated cerebellar cultures and immunoelectron microscopy of the adult rat cerebellum. Combined, our results indicate that eEF1A is associated with the PSD.
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