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[Establishment of a sandwich ELISA for detecting serum sTRAIL and its application]
Ying-Na Li1, Yi Li, Xue-Song Liu
1Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.
Aim:
To establish quantitative ELISA for detecting souble TRAIL and to evaluate its clinical application.
Methods:
The mAb FMU1.1 against TRAIL was used as coating antibody, and the rabbit polyclonal antibody to TRAIL as a sandwich antibody. The serum sTRAIL levels of nine patients with hemorrhagic fever with renal syndrome and forty psoriasis patients were measured by this method. RESUITS: Sandwich ELISA was established by combination of mAb and pAb. The sensitivity of the ELISA was 0.08 microg/L. The increased levels of serum sTRAIL were found in three cases out of nine HFRS patients and eight cases out of forty psoriasis patients.
Conclusion:
A sandwich ELISA is developed, which provide an useful tool judging the patients condition, curable effect and prognosis of some related diseases.
