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[Inducible expression of reconstructed human caspase-6 gene in Hela cells]
Yan-Ming Xu1, Rui Liu, Chun-Xu Hai
1Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an 710032,China.
Aim:
To observe the pro-apoptotic effect of RCasp-6 gene in Hela cells.
Methods:
RCasp-6 gene was amplified by PCR and cloned into the pIND vector. Hela cells were transfected with pIND-RCasp-6 and then inducced with ecdyson analogue. Expression of target gene was detected by immunocytochemical staining. Changes of the morphology and growth of Hela cells subjected to transfection of target gene were observed by HE staining and viable cell counting.
Results:
RCasp-6 gene was cloned by PCR and its eukaryotic expression vector was successfully reconstructed. Expression of RCasp-6 gene in the transfected Hela cells leads to the morphological changes of the cells. Many of the transfected Hela cells shrunk and some cells were died.
Conclusion:
The expression of reconstructed human caspase-6 gene can efficiently accelerate death of Hela cells.
