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[Screening of TNF-alpha mimotopes from c7c phage display peptide library]
Hai-Bo Luo1, Bei-Yi Liu, Yan-Jun Liu
1Department of Immunology, First Military Medical University, Guangzhou 510515, China.
Aim:
To acquire oligo-peptides mimicking TNF-alpha epitopes.
Methods:
The TNF-alpha mimotopes were screened from c7c phage display peptide library by using neutralizing mAb J1D9 against TNF-alpha, and positive clones were identified by sandwich ELISA.
Results:
9 out of 20 phage clones were identified as positive clones which can bind to mAb J1D9. The deduced amino acid sequence analysis showed three different sequences: c-RRPAQSG-c, c-NKHNRKI-c and c-RGMSRKI-c.
Conclusion:
All of the three peptides obtained can mimic the epitopes of TNF-alpha and bind to mAb J1D9.
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