Related Experiment Video
Updated: Aug 24, 2026
Preparation of Tumor Antigen-loaded Mature Dendritic Cells for Immunotherapy
Published on: August 1, 2013
[Study on immunoreaction induced by DCs loaded with hepatocarcinoma antigen peptide in-vitro]
Ping Qu1, Yan-Fang Sui, Ai-Lin Guo
1Department of Pathology, Fourth Military Medical University,Xi'an 710032 China.
Aim:
To investigate the potency and antitumor effect of specific cytotoxic T lymphocytes induced by hepatocarcinoma antigen peptide EPVTKAEML-loaded dendritic cells (DCs).
Methods:
The healthy donors of HLA-B7 genotype were selected by PCR-SSP. Isolated and cultured DCs from HLA-B7 donors' spleen tissues were loaded with the peptide EPVTKAEML from hepatocarcinoma cell line HHCC. The specific CTL reaction and inhibitory effect of anti-HLA-I molecule mAb on CTL cytotoxicity were detected in-vitro by (51)Cr-release assay.
Results:
4 donors with HLA-B7 genotype were found. Their spleen T lymphocytes could be induced to proliferate and differentiate into specific CTLs by DCs loaded with the peptide. Antibody-blocking test demonstrated that the cytotoxity of CTLs was strictly limited by MHC-I molecule.
Conclusion:
EPVTKAEML-loaded DCs can induce efficient and specific antitumor immune reaction in-vitro.
