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Updated: Aug 24, 2026

Supramaximal Intensity Hypoxic Exercise and Vascular Function Assessment in Mice
Published on: March 15, 2019
Ischemic preconditioning improves oxygenation of exercising muscle in vivo
Taketo Saito1, Takashi Komiyama, Haruo Aramoto
1Department of Surgery, Graduate School of Medicine, The University of Tokyo, Tokyo 113-8655, Japan. taketo@sage.ocn.ne.jp
Background:
Ischemic preconditioning (IP) improves tissue tolerance to prolonged ischemia. In this study, we investigated the functional effect of IP on skeletal muscle of rat hind limb by means of near-infrared spectroscopy (NIRS) and by measuring myeloperoxidase (MPO) activity.
Materials And Methods:
Adult male Sprague Dawley rats were divided into four separate protocol groups according to different preparations prior to 2 h of global ischemia: a group of ischemic reperfusion without any preparation (I/R), ischemic reperfusion with ischemic preconditioning (IP+IR), ischemic reperfusion with adenosine infusion (ADO+I/R), and sham operation. Ischemia and ischemic preconditioning were induced by clamping infrarenal abdominal aorta and left common iliac artery. For each rat, an exercise test of gastrocnemius muscles was performed by stimulating sciatic nerve before and after global ischemia while performing NIRS. MPO activity of ischemic muscles was also measured.
Results:
Half-resaturation time after exercise and MPO activity were significantly improved in IP+IR and ADO+I/R groups. Difference of oxyhemoglobin during exercise was also improved in the IP+IR group.
Conclusion:
This study has demonstrated that IP provides the protective effect on in vivo skeletal muscle oxygenation during exercise.
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