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Determination of baicalin and baicalein in rat plasma by high-performance liquid chromatography with electrochemical
Y Wakui1, E Yanagisawa, E Ishibashi
1Research Institute for Pharmacology, Tsumura & Co., Ibaraki, Japan.
Journal of Chromatography
|March 13, 1992
Abstract:
A rapid and sensitive method, using electrochemical detection, has been developed for the determination of baicalin and baicalein, the flavonoid of Scutellariae radix, in rat plasma. Following separation by high-performance liquid chromatography, baicalin and baicalein were oxidized at a glassy carbon electrode to permit selective electrochemical detection. Absolute detection limits were found to be 5 ng/ml from 50 microliters of plasma for baicalin and 2 ng/ml from 100 microliters of plasma for baicalein. The resulting assays were suitable for pharmacokinetic studies of baicalin and baicalein in rats.