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Chiral cyclodextrin-modified microemulsion electrokinetic chromatography.

Melissa D Mertzman1, Joe P Foley

  • 1Department of Chemistry, Drexel University, Philadelphia, PA, USA.

Electrophoresis
|June 3, 2004
PubMed
Summary
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This study introduces cyclodextrin-modified microemulsion electrokinetic chromatography (CD-MEEKC) for chiral separations. Optimized conditions achieved high resolution for enantiomers, including levetiracetam, previously unseparated by capillary electrophoresis.

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Chiral Separations

Background:

  • Cyclodextrin (CD)-modified microemulsion electrokinetic chromatography (CD-MEEKC) is a novel technique.
  • CD-MEEKC has not been previously applied to chiral separations.

Purpose of the Study:

  • Investigate the application of CD-MEEKC for chiral separations.
  • Explore various microemulsions with CD additives.
  • Optimize separation parameters for enhanced resolution.

Main Methods:

  • Two microemulsions were explored: ethyl acetate sodium dodecyl sulfate and chiral dodecoxycarbonylvaline (DDCV).
  • Microemulsions were paired with neutral (hydroxypropyl-beta-CD) and anionic (sulfated-beta-CD) cyclodextrins.
  • The chiral DDCV microemulsion was tested in both R- and S- forms.

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  • Parameters like buffer system, applied voltage, surfactant enantiomer, and CD type were varied.

    • Main Results:

    • Dramatic improvements in chiral separations were observed by varying parameters.
    • Resolution was highly dependent on buffer identity and concentration.
    • Resolution ranged from 0.8 to 4.8, with plate counts from 4000 to 26,000.
    • S- and R-levetiracetam were successfully separated in under 8 minutes with a resolution of 1.1.

    Conclusions:

    • CD-MEEKC is effective for chiral separations.
    • Optimization of parameters significantly enhances separation resolution.
    • This method enables the enantioseparation of compounds like levetiracetam, previously challenging for capillary electrophoresis.