[Establishment and characterization of human alloantigen induced T cell clones]

To estabilish and analyze stable human T cell clones cultured in-vitro.

The activated T cells from mixed lymphocyte culture (MLC) were cloned by limiting dilution using irradiated PBMCs as feeder cells. T cell clones were then characterized by immunofluorescence staining and flow cytometry analysis.

16 clones were obtained, of which all but one were alphabetaT cells. Among the 15 alphabetaT cell clones, 9 clones belonged to Th subset, including 3 Th0 and 6 Th2, and 6 clones belonged to Tc subset, including 5 Tc0 and 1 Tc2.

Fifteen human T cell clones were successfully established, which lays the foundation for study on markers and function of Tc1 and Tc2 subsets.