[Modified nested polymerase chain reaction in one tube for detection of Enterovirus]

A method of a modified nest-type polymerase chain reaction (MN-PCR), made in one tube, was elaborated that enhances the sensitivity and cuts the risk of cross-contamination in enteroviruses (EV) detection. The method, as described in detail above, was used to detect EV RNA in 76.9% and 31.25% of examined autopsy samples (13 liquor and 16 cardiac-tissue samples, respectively). It enabled the detection of EV RNA in 6.25% of samples that used to be negative, when tested by MN-PCR in 2 tubes. MN-PCR with one tube is a reliable, sensitive and specific diagnostic tool; it can be recommended for the routine diagnostics of enterovirus infection.