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Updated: Aug 23, 2026
A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses
Published on: August 31, 2014
[Study on the immunogenicity of HIV-1 gag vaccine]
Wen-Zheng Jiang1, Ning-Yi Jin, Zi-Jian Li
1Key Laboratory of Genetic Engineering, Quartermaster University of PLA, Changchun 130062, China. wzjiang@hotmail.com
Aim:
To detect the immunogenicity of HIV-1 gag DNA vaccine.
Methods:
The serum antibody titers of the immunized mice were detected by ELISA. The numbers of spleen's CD4(+) and CD8(+) T cells were analyzed by the fluorescent antibody staining and the cytotoxic activity was determined by lactate dehydrogenase (LDH) release assay.
Results:
The titers of the serum antibodies and the numbers and cytotoxic activity of spleen's T lymphocyte subsets had significant differences (P<0.05 and P<0.01, respectively) between pVAXGAG and pVAX1 vaccinated groups.
Conclusion:
HIV-1 DNA vaccine plasmid pVAXGAG could elicit both the specific humoral and cellular immunities in BALB/c mice.
