Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enthalpy arrays.

Francisco E Torres1, Peter Kuhn, Dirk De Bruyker

  • 1Scripps-PARC Institute for Advanced Biomedical Sciences, Palo Alto Research Center, 3333 Coyote Hill Road, Palo Alto, CA 94304, USA.

Proceedings of the National Academy of Sciences of the United States of America
|June 24, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tissue removal inside the beating heart using a robotically delivered metal MEMS tool.

The International journal of robotics research·2026
Same author

Decoupling of global metabolic flux and proteome partitioning in bacteria.

Science (New York, N.Y.)·2026
Same author

Quantifying vocal subsystems of adults with minimally verbal autism spectrum disorder.

Frontiers in human neuroscience·2026
Same author

Wearable Tracking of Eye and Body Movements During Breaching Training: Toward Real-Time Blast Injury Monitoring.

Journal of neurotrauma·2026
Same author

Untargeted Multiple Reaction Monitoring.

Analytical chemistry·2026
Same author

Speech Acoustic Markers Can Detect Mild Cognitive Impairment in Parkinson's Disease.

IEEE journal of selected topics in signal processing·2026
Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Researchers developed novel enthalpy arrays for universal molecular interaction detection, offering a faster, more sensitive method than traditional techniques. This innovation simplifies assays for protein-ligand binding, enzyme activity, and cellular respiration.

Area of Science:

  • Biophysical Chemistry
  • Assay Development
  • Microscale Technology

Background:

  • Conventional microcalorimetry requires extensive assay development, such as fluorescent labeling or reagent immobilization, which can interfere with molecular interactions.
  • There is a need for universal assay methodologies that minimize sample requirements and measurement times while preserving the integrity of molecular interactions.

Purpose of the Study:

  • To report the fabrication of microscale enthalpy arrays.
  • To demonstrate the utility of these arrays for detecting diverse molecular interactions, including protein-ligand binding, enzymatic turnover, and mitochondrial respiration.
  • To establish enthalpy arrays as a universal assay methodology.

Main Methods:

  • Fabrication of 96-detector enthalpy arrays utilizing microscale technology.

Related Experiment Videos

  • Application of enthalpy arrays to measure protein-ligand binding (RNase A + cytidine 2'-monophosphate; streptavidin + biotin).
  • Utilizing enthalpy arrays to quantify enzymatic turnover (glucose phosphorylation by hexokinase) and mitochondrial respiration (in the presence of 2,4-dinitrophenol).

    • Main Results:

    • Enthalpy arrays provide a universal assay methodology, eliminating the need for specific assay development.
    • Microscale fabrication enables high-density arrays (96 detectors) with reduced sample quantity and measurement time compared to conventional microcalorimetry.
    • Successful detection of various molecular interactions, including specific protein-ligand pairs, enzyme kinetics, and mitochondrial respiratory activity.

    Conclusions:

    • Enthalpy arrays represent a significant advancement in assay technology for studying molecular interactions.
    • The universal and label-free nature of enthalpy arrays simplifies experimental design and reduces potential artifacts.
    • This technology offers a powerful, miniaturized platform for diverse biochemical and cellular analyses.