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Protein Data Bank depositions from synchrotron sources
Jiansheng Jiang1, Robert M Sweet
1Biology Department, Brookhaven National Laboratory, USA. jiang@bnl.gov
Journal of Synchrotron Radiation
|June 24, 2004
Summary
Synchrotron radiation facilities are increasingly used for X-ray crystallography, with over 75% of Protein Data Bank depositions utilizing them by 2003. These sources enable solving larger protein structures with high quality.
Area of Science:
- Structural Biology
- Biophysics
- Crystallography
Background:
- Protein Data Bank (PDB) archives structural data.
- International synchrotron radiation facilities are crucial for macromolecular crystallography.
Purpose of the Study:
- To analyze PDB depositions from synchrotron sources.
- To assess the impact of synchrotron radiation on X-ray structure determination.
Main Methods:
- Survey and analysis of PDB entries.
- Utilized a web-based database synchronized with PDB releases.
- Examined statistics on experimental data quality and model refinement.
Main Results:
- Since 1999, synchrotron use for X-ray structures exceeded 50% annually, reaching 75% by 2003.
- Synchrotron sources facilitate solving larger protein structures.
- Structures solved using synchrotrons demonstrate equivalent or superior quality compared to home sources.
Conclusions:
- Synchrotron radiation is vital for advancing structural biology.
- Synchrotron sources enhance the capability to determine complex and large biomolecular structures.