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Updated: Aug 23, 2026

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Optimizing Tubulin Yield from Porcine Brain Tissue
Published on: October 11, 2024
Total synthesis and anti-tubulin activity of epi-c3 analogues of cryptophycin-24
Suzanne B Buck1, Jacquelyn K Huff, Richard H Himes
1Department of Medicinal Chemistry, University of Kansas, 1251 Wescoe Drive, Lawrence, KS 66045-7582, USA.
Journal of Medicinal Chemistry
|June 25, 2004
Abstract:
Epi-C3-cryptophycin-24, epi-C3-m-chlorobenzyl-cryptophycin-24, and the corresponding styrenes were synthesized and tested in vitro against the MCF-7 and multidrug-resistant MCF-7/ADR breast cancer cell lines and in an in vitro tubulin assembly assay. The results demonstrate that the S configuration at the C3 stereocenter is not required to induce potent cytotoxicity and the m-Cl substituent present on the C10 side chain did not induce any large change in activity.
