Related Experiment Video
Updated: Aug 23, 2026

Extraction and Visualization of Protein Aggregates after Treatment of Escherichia coli with a Proteotoxic Stressor
Published on: June 29, 2021
Stress induced by recombinant protein production in Escherichia coli
1Institut für Biotechnologie, Martin-Luther-Universität Halle-Wittenberg, Kurt-Mothes-Strasse 3, 06120 Halle, Germany.
Abstract:
Strong production of recombinant proteins interferes with cellular processes in many ways. Drainage of precursors and energy urges the cell to readjust metabolic fluxes and enzyme composition, stress responses are induced, and hence the cellular activity is shifted from growth to reorganisation of biomass. This may result in inhibition of growth or low level of product accumulation. The extent of the bacterial stress response is determined by the specific properties of the recombinant protein, and by the rates of transcription and translation. Taking into account the capacities of the host for protein processing and physiological adaptation, production schemes can be developed that enhance volumetric productivity and sustainability of the process.
Related Concept Videos
Stringent Response in E. coli
Production of Pharmaceuticals
Upstream Processing
Other Stress Responses in Bacteria

