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Updated: Aug 23, 2026

Construction of Model Lipid Membranes Incorporating G-protein Coupled Receptors (GPCRs)
Published on: February 5, 2022
The ArfGAP Glo3 is required for the generation of COPI vesicles
Stephen M Lewis1, Pak Phi Poon, Richard A Singer
1Departments of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 1X5.
Abstract:
The small GTPase Arf and coatomer (COPI) are required for the generation of retrograde transport vesicles. Arf activity is regulated by guanine exchange factors (ArfGEF) and GTPase-activating proteins (ArfGAPs). The ArfGAPs Gcs1 and Glo3 provide essential overlapping function for retrograde vesicular transport from the Golgi to the endoplasmic reticulum. We have identified Glo3 as a component of COPI vesicles. Furthermore, we find that a mutant version of the Glo3 protein exerts a negative effect on retrograde transport, even in the presence of the ArfGAP Gcs1. Finally, we present evidence supporting a role for ArfGAP protein in the generation of COPI retrograde transport vesicles.
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