Fluorometric assay for determining epoxide hydrolase activity
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Updated: Aug 23, 2026
Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
1Institute for Technical Biochemistry, University of Stuttgart, Allmandring 31, D-70569, Germany.
Abstract:
We have developed a rapid screening procedure that enables the screening of hundreds of enzyme samples or variants for epoxide hydrolase activity towards any substrate. The procedure detects the products of the enzymatic reaction via periodate cleavage and remaining fluorescence of carboxyfluorescein.
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