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Technology-enabled knowledge translation: frameworks to promote research and practice

Kendall Ho1, Ralph Bloch, Tunde Gondocz

  • 1Department of Surgery, Division of Continuing Medical Education, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia.

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Improving Translational Accuracy02:07

Improving Translational Accuracy

Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...