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In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers
Published on: July 28, 2018
Structural organization of DMPC lipid layers on chemically micropatterned self-assembled monolayers as biomimetic
A Brechling1, M Pohl, U Kleineberg
1Faculty of Physics, University of Bielefeld, Universitaetsstr. 25, D-33501 Bielefeld, Germany. armin.brechling@uni-bielefeld.de
Abstract:
The growth structure of DMPC lipid layers on hydrophobic and hydrophilic alkylsilane-based self-assembled monolayers adsorbed on silicon has been investigated by means of X-ray reflectometry and atomic force microscopy. Hydrophilic modification of hydrophobically terminated ODS-SAMs has been achieved by dose-controlled irradiation with DUV light. While island formation of small DMPC bilayer islands is observed on hydrophobic SAM surfaces, closed layers of DMPC monolayers are formed on hydrophilic SAM surfaces. Furthermore, DMPC adsorption on chemically micropatterned substrates with alternating hydrophobic/hydrophilic surface properties has been studied by imaging ellipsometry and photoemission microscopy. Indication for at least partial bridging of hydrophobic areas by an adsorbed DMPC monolayer has been found.
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