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Updated: Aug 4, 2026

Detection of DNA Double-Stranded Breaks in Mouse Oocytes
Published on: June 23, 2023
Downregulation of peroxiredoxin V stimulates formation of etoposide-induced double-strand DNA breaks
Andrei V Kropotov1, Pavel S Grudinkin, Nadezha M Pleskach
1Institute of Cytology, Russian Academy of Sciences, Tikchoretskii Av.4, 194064 St. Petersburg, Russia.
Abstract:
Antioxidant protein Peroxiredoxin V (PrxV) is located in mitochondria and peroxisomes but is also present in the nucleus. Here, we show that nuclear PrxV associates with coilin-containing bodies suggesting possible interaction of this protein with transcription complexes. We also studied etoposide-induced phosphorylation of histone H2AX (gamma-H2AX) in human cells in which PrxV activity was downregulated (knockdown, KD-clones) or compromised by overexpression of redox-negative (RD) protein. In KD clones, but not in RD-clones, formation of etoposide-induced gamma-H2AX was increased, indicating that PrxV inhibits conversion of topoisomerase II cleavage complexes into double-strand DNA breaks but this inhibition is not caused by its antioxidant activity.
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