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Updated: Aug 22, 2026

03:53
Development of Multiplex Real-Time RT-qPCR Assays for the Detection of SARS-CoV-2, Influenza A/B, and MERS-CoV
Published on: November 10, 2023
SARS coronavirus detection
Andreas Nitsche1, Brunhilde Schweiger, Heinz Ellerbrok
1Robert Koch-Institut, Berlin, Germany. nitschea@rki.de
Emerging Infectious Diseases
|August 25, 2004
Abstract:
We developed a set of three real-time reverse transcription-polymerase chain reaction (PCR) assays that amplify three different regions of the SARS-associated coronavirus (SARS-CoV), can be run in parallel or in a single tube, and can detect <10 genome equivalents of SARS-CoV. The assays consider all currently available SARS-CoV sequences and are optimized for two prominent real-time PCR platforms.
