Related Experiment Video

Updated: Aug 22, 2026

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
13:08

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Published on: September 9, 2012

[Detection of coagulation factor V in patients with severe hepatitis and its clinical significance]

Zheng-sheng Zou1, Zhi-guo Liu, Ju-mei Chen

  • 1The No. 302 Hospital of PLA, Beijing 100039, China.

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi = Zhonghua Shiyan He Linchuang Bingduxue Zazhi = Chinese Journal of Experimental and Clinical Virology
|September 2, 2004
PubMed

Insights

Coagulation factor V (CFV) levels are crucial prognostic indicators in severe hepatitis, offering greater specificity than prothrombin activity (PTA). Monitoring both CFV and PTA aids in earlier, more accurate diagnosis and may inform liver transplant criteria.

Area of Science:

  • Hepatology
  • Hematology
  • Clinical Diagnostics

Background:

  • Severe hepatitis poses significant diagnostic and prognostic challenges.
  • Coagulation factor V (CFV) and prothrombin activity (PTA) are key hemostatic markers.
  • The prognostic value of CFV in severe hepatitis requires further elucidation.

Purpose of the Study:

  • To investigate the prognostic significance of coagulation factor V (CFV) levels in severe hepatitis.
  • To evaluate the role of CFV in clinical diagnostic criteria for severe hepatitis.
  • To compare the diagnostic and prognostic utility of CFV with prothrombin activity (PTA).

Main Methods:

  • 129 CFV and PTA measurements were performed on 58 severe hepatitis patients.
  • Turbidimetry was used for CFV and PTA level testing.
  • Statistical analysis (SPSS, SDAS) was employed for comparative studies and clinical significance assessment.

Main Results:

  • CFV and PTA levels at disease onset were 15.3%±9.7% and 23.5%±10.0%, respectively.
  • Decreasing CFV or PTA levels correlated with increased mortality in severe hepatitis (P=0.000).
  • CFV and PTA levels decreased in non-survivors and increased in survivors, with PTA changes preceding CFV changes.

Conclusions:

  • CFV level serves as a vital prognostic indicator in severe hepatitis, demonstrating higher specificity than PTA.
  • Simultaneous measurement of CFV and PTA can enhance early and accurate diagnosis of severe hepatitis.
  • Further research into using CFV as a criterion for liver transplantation in severe hepatitis patients is recommended.
Abstract

More Related Videos

Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA04:11

Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA

Published on: December 15, 2023

Extracellular Vesicle Tissue Factor Activity Assay03:53

Extracellular Vesicle Tissue Factor Activity Assay

Published on: December 29, 2023

Related Concept Videos

Venous Thrombosis II: Clinical Manifestations and Diagnostic Studies01:20

Venous Thrombosis II: Clinical Manifestations and Diagnostic Studies

The key difference between Superficial Vein Thrombosis (SVT) and Deep Vein Thrombosis (DVT) lies in their location and severity.Clinical ManifestationsSVT typically presents with localized pain, tenderness, and redness along the course of a superficial vein, often accompanied by a palpable, cord-like structure under the skin. This condition is usually less dangerous than DVT but can be uncomfortable and may lead to complications such as cellulitis or, rarely, a clot extension into the deep...
Viral Hepatitis I: Introduction01:28

Viral Hepatitis I: Introduction

Viral hepatitis is an inflammatory condition of the liver caused by infection with hepatotropic viruses, most commonly hepatitis A, B, C, D, and E. Despite variations in structure and transmission, all viruses mentioned infect hepatocytes and provoke immune responses that can hinder liver function. Additionally, some non-hepatotropic viruses can also lead to hepatic inflammation.Hepatitis A VirusHepatitis A virus (HAV) is transmitted through the fecal–oral route, typically by ingestion of food...
Venous Thrombosis III: Interprofessional Care01:29

Venous Thrombosis III: Interprofessional Care

Venous thrombosis requires effective prevention and treatment strategies to improve patient outcomes and reduce potential complications.Prevention StrategiesHealthcare providers must prioritize preventing venous thromboembolism (VTE) for all adult patients upon admission. Interventions depend on bleeding and thrombosis risk, medical history, current medications, diagnoses, planned procedures, and patient preferences. Patients on bed rest should change positions every two hours and, if not...
Hepatitis01:25

Hepatitis

Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver. The...
Cirrhosis II: Pathophysiology01:24

Cirrhosis II: Pathophysiology

Cirrhosis is a progressive chronic liver injury caused by prolonged inflammation, excessive fibrotic remodeling, and impaired regeneration. Over time, repeated hepatic insults disrupt the liver’s architecture and function, leading to reduced blood flow, impaired bile drainage, and diminished metabolic capacity.Pathophysiology of cirrhosisCirrhosis arises from three main responses to chronic liver damage: inflammation, immune activation, and hepatocyte death. These processes lead to structural...
Rheumatic Heart Disease II: Clinical Manifestations and Diagnostic Studies01:22

Rheumatic Heart Disease II: Clinical Manifestations and Diagnostic Studies

The key clinical manifestations of Rheumatic heart disease (RHD) include several distinct cardiac symptoms.Carditis, a hallmark of acute rheumatic fever, involves inflammation of the heart's endocardium, myocardium, and pericardium. Chronic RHD often results from recurrent episodes of carditis. Its symptoms include the following:Murmurs are caused by valvular damage, especially to the mitral and aortic valves. Mitral stenosis or regurgitation is common, with characteristic heart murmurs...