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Updated: Dec 18, 2025

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
Published on: January 20, 2022
Performance of quantitative analyses by liquid chromatography-electrospray ionisation tandem mass spectrometry: from
1Nestlé Research Centre, Nestec Ltd., Vers-chez-les-Blanc, 1000, Lausanne 26, Switzerland. thierry.delatour@rdls.nestle.com
Abstract:
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a versatile coupling system which combines both selectivity and sensitivity and certainty. Hence, it is generally considered as the most reliable technique to quantify chemical compounds in complex matrices. In the present paper, we evaluate the performance of LC-MS/MS methods for the quantification of 3-nitrotyrosine in human urine in order to point out its dependence on the design of the quantification method, and emphasize the role of matrix effects in the performance. We compare external and internal calibrations, isotope dilution and isotopomer-based exact matching. The role of both sample preparation and multiple transitions monitoring is particularly addressed.
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