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Updated: Aug 22, 2026

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Detection of Bacteria Using Fluorogenic DNAzymes
Published on: May 28, 2012
Fluorogenic selective and differential medium for isolation of Enterobacter sakazakii
1Department of Food Science and Human Nutrition, Washington State University, Pullman, WA 99164-6376, USA.
Applied and Environmental Microbiology
|September 4, 2004
Abstract:
4-Methylumbelliferyl-alpha-D-glucoside, the fluorogenic substrate of alpha-glucosidase, was used as a selective marker to develop a differential medium for Enterobacter sakazakii. This bacterium showed strong fluorogenic characteristics clearly distinguishable from other microorganisms. On the basis of reducing background noise, an optimum basal medium and nitrogen source were selected. Incubation conditions were optimized.

