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Exercise normalises overexpression of TNF-alpha in knockout mice
Charlotte Keller1, Pernille Keller, Mercé Giralt
1Copenhagen Muscle Research Centre, Rigshospitalet, Denmark. charlotte_keller@yahoo.com
Biochemical and Biophysical Research Communications
|September 11, 2004
Summary
A single hour of exercise can normalize tumor necrosis factor-alpha (TNF-alpha) gene expression, thereby combating insulin resistance. This effect may involve interleukin-6 (IL-6) or independent pathways.
Area of Science:
- Exercise physiology
- Immunometabolism
- Molecular biology
Background:
- Tumor necrosis factor-alpha (TNF-alpha) is implicated in insulin resistance, with elevated levels observed in metabolically challenged individuals.
- TNF-alpha inhibits insulin receptor signaling, but the factors regulating its overexpression remain unclear.
Purpose of the Study:
- To investigate the impact of a 1-hour exercise session on TNF-alpha gene expression in mice lacking TNF receptors (TNFR1/2).
- To explore the role of interleukin-6 (IL-6) in mediating exercise-induced regulation of TNF-alpha.
Main Methods:
- Utilized TNF receptor 1 and 2 knockout mice and IL-6 knockout mice.
- Administered a 1-hour exercise intervention.
- Measured TNF-alpha gene expression in muscle tissue.
Main Results:
- TNF-alpha gene expression was initially overexpressed in muscle of TNFR knockout mice.
- Exercise normalized TNF-alpha overexpression in TNFR knockout models.
- A modest decrease in TNF-alpha was observed in IL-6 knockout mice post-exercise.
Conclusions:
- A single bout of exercise can regulate TNF-alpha-induced insulin resistance by normalizing TNF-alpha expression.
- Exercise-mediated regulation of TNF-alpha may involve IL-6, but an IL-6 independent mechanism also appears to exist.