[Morpho-functional changes of splenic autotransplants at the different time intervals after the operation]

This investigation was aimed at the study of splenic autotransplant cytoarchitectonics and cellular structures functional activity 3-60 days after the operation. The studies were carried out in 70 albino male rats including intact animals (n-10) and rats subjected to splenectomy and subsequent spleen heterotopic autotransplantation (n-60). Cryostat sections of transplants were processed for Falc and Cross luminescent-histochemical methods and were stained with hematoxylin and eosin. Biogenic amines content was measured using LUMAM-4 microscope with FMEL-1A spectrofluorimetric device. The number of luminescent cells in periarterial T-dependent zone was found to increase significantly after 7 days, while the unchanged lymphoid nodules were found in the zone of unaltered tissues. At day 15 "intrafollicular" luminescent granulocytes appeared in the newly formed lymphoid nodules. At days 15 to 45 the increased content of biogenic amines was detected in the "intrafollicular" cells, while the significant rise in the amount of amines was seen in the lymphocytes of nodule T-independent zone. It is suggested that the presence of intact nodules in transplant peripheral zone together with an increase in cell numbers in T-dependent zone, are the key events in the development of immune-correction effect of the transplanted spleen during the first two weeks after the operation.