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Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts
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Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

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Bringing the role of mRNA decay in the control of gene expression into focus

Carol J Wilusz1, Jeffrey Wilusz

  • 1Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA. cwilusz@colostate.edu

Trends in Genetics : TIG
|September 15, 2004
PubMed

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The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
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Regulation of Expression Occurs at Multiple Steps02:24

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Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...