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Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
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[Purification of recombinant humanized anti-HBsAg Fab antibody by affinity chromatography]

Ning Deng1, Wen-yin Chen, Jun-jian Xiang

  • 1Lab. of Molecular Immunology and Antibody Engineering, Life Sciences and Technology College, Jinan University, Guangzhou 510632, China. tdengn@jnu.edu.cn

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology
|September 16, 2004
PubMed

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Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...