Search research articles

Search research articles

Related Experiment Videos

Mouse gamete adhesion molecules.

P M Wassarman¹

¹Department of Cell and Developmental Biology, Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110.

Biology of Reproduction

|February 1, 1992

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Program of early development in the mammal: synthesis and intracellular migration of histone H4 during oogenesis in the mouse.

Developmental biology·2010

Same author

Egg extracellular coat proteins: from fish to mammals.

Histology and histopathology·2006

Same author

Mouse zona pellucida genes and glycoproteins.

Cytogenetic and genome research·2004

Same author

Multiple functions of mouse zona pellucida glycoprotein mZP3, the sperm receptor.

Italian journal of anatomy and embryology = Archivio italiano di anatomia ed embriologia·2001

Same author

Association of egg zona pellucida glycoprotein mZP3 with sperm protein sp56 during fertilization in mice.

The International journal of developmental biology·2001

Same author

Secretion of mouse ZP3, the sperm receptor, requires cleavage of its polypeptide at a consensus furin cleavage-site.

Biochemistry·2001

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Mouse sperm and egg adhesion molecules mediate species-specific fertilization. Key proteins like mZP3 and sp56 facilitate sperm binding and the acrosome reaction, preventing polyspermy.

Area of Science:

Reproductive Biology
Cellular and Molecular Biology
Biochemistry

Background:

Sperm-egg interactions are crucial for fertilization and are mediated by complementary adhesion molecules on gamete surfaces.
These interactions are species-specific and initiate events leading to successful fertilization.
Post-fertilization modifications of these molecules prevent polyspermy.

Purpose of the Study:

To identify and characterize the specific adhesion molecules involved in mouse gamete recognition and binding.
To elucidate the roles of these molecules in mediating species-specific fertilization and preventing polyspermy.
To investigate the carbohydrate-mediated nature of sperm-egg adhesion.

Main Methods:

Identification and characterization of mouse egg zona pellucida glycoproteins (mZP3, mZP2) and sperm surface proteins (sp56).

Related Experiment Videos

Analysis of carbohydrate-mediated binding interactions between sperm and egg molecules.

Investigation of sperm acrosome reaction induction upon binding to egg zona pellucida.

Main Results:

Mouse egg zona pellucida glycoprotein mZP3 acts as the primary sperm receptor, with sperm binding mediated by O-linked oligosaccharides.
Sperm protein sp56 is a putative egg-binding protein that recognizes mZP3 oligosaccharides.
Acrosome-reacted sperm bind to mZP2 (secondary sperm receptor) on the zona pellucida.

Conclusions:

Fertilization in mice involves a complex interplay of specific egg and sperm surface adhesion molecules.
Carbohydrate recognition of mZP3 by sperm is critical for initiating the acrosome reaction and subsequent fertilization.
mZP2 serves as a secondary receptor for acrosome-reacted sperm, ensuring proper binding to the egg.