Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Functional engineered channels and pores (Review).

Hagan Bayley1, Lakmal Jayasinghe

  • 1Department of Chemistry, University of Oxford, Oxford OX1 3TA, UK. hagan.bayley@chem.ox.ac.uk

Molecular Membrane Biology
|September 17, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Astrocyte Enrichment of 3D Cortical Constructs Enhances Brain Repair.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Human-derived cardiac-neural microtissues reveal catecholaminergic polymorphic ventricular tachycardia is also a disease of the sympathetic neuron.

The Journal of physiology·2026
Same author

Targeted high-resolution sensing of volatile organic compounds by covalent nanopore detection.

Nature communications·2025
Same author

High-Resolution Patterned Delivery of Chemical Signals From 3D-Printed Picoliter Droplet Networks.

Advanced materials (Deerfield Beach, Fla.)·2025
Same author

Rational Design Principles for <i>De Novo</i> α-Helical Peptide Barrels with Dynamic Conductive Channels.

Journal of the American Chemical Society·2025
Same author

3D Microtumors Representing Ovarian Cancer Minimal Residual Disease Respond to the Fatty Acid Oxidation Inhibitor Perhexiline.

Advanced healthcare materials·2025
Same journal

Correction.

Molecular membrane biology·2020
Same journal

Potassium channels and their role in glioma: A mini review.

Molecular membrane biology·2020
Same journal

Spatial organization of palmitoyl acyl transferases governs substrate localization and function.

Molecular membrane biology·2020
Same journal

Uncoupling protein 2 prevents ischaemia reperfusion injury through the regulation ROS/NF-κB signalling in mice.

Molecular membrane biology·2019
Same journal

Zinc uptake in the Basidiomycota: Characterization of zinc transporters in <i>Ustilago maydis</i>.

Molecular membrane biology·2019
Same journal

The KdpFABC complex - K<sup>+</sup> transport against all odds.

Molecular membrane biology·2019
See all related articles

Membrane protein engineering has advanced through scaffold redesign and improved assembly techniques. Future progress relies on structural data, computational methods, and applications in therapeutics and sensors.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Protein Engineering

Background:

  • Significant advancements in membrane protein engineering over the past five years.
  • Engineering efforts primarily focused on redesigning existing protein scaffolds.
  • Established techniques include genetic engineering, chemical modifications, and total synthesis.

Purpose of the Study:

  • To review recent progress in membrane protein engineering.
  • To highlight underutilized techniques like combinatorial mutagenesis and directed evolution.
  • To discuss the emerging era of membrane protein engineering driven by new technologies and data.

Main Methods:

  • Genetic engineering (covalent and non-covalent modifications)
  • Unnatural amino acid mutagenesis

Related Experiment Videos

  • Total synthesis via chemical ligation
  • Improvements in assembling and purifying heteromeric multisubunit pores

    • Main Results:

    • Redesign of existing scaffolds is the dominant engineering approach.
    • Combinatorial mutagenesis and directed evolution are underutilized.
    • De novo design of channels and pores has progressed more slowly.
    • Significant improvements in assembling and purifying complex protein structures.

    Conclusions:

    • A new era in membrane protein engineering is beginning.
    • This era is fueled by increased structural information and understanding of protein dynamics.
    • Technical improvements in refolding and computational approaches are key drivers.
    • Future applications in therapeutics and sensor technology are anticipated.