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Circular box C/D RNAs in Pyrococcus furiosus.

Natalia G Starostina1, Sarah Marshburn, L Steven Johnson

  • 1Department of Biochemistry and Molecular Biology, University of Georgia, Davison Life Science Building, Athens, GA 30602, USA.

Proceedings of the National Academy of Sciences of the United States of America
|September 18, 2004
PubMed
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Box C/D RNAs, crucial for RNA modification, were discovered as circular molecules in the archaeon Pyrococcus furiosus. This finding reveals a novel RNA biogenesis pathway in archaea.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Archaea Biology

Background:

  • Box C/D RNAs are essential small, noncoding RNAs involved in RNA modification across eukaryotes and archaea.
  • Their known forms are typically linear RNA molecules.

Purpose of the Study:

  • To investigate the forms of box C/D RNAs in the hyperthermophilic archaeon Pyrococcus furiosus.
  • To explore potential novel biogenesis pathways for these RNAs.

Main Methods:

  • Northern blot analysis to detect and differentiate RNA species.
  • Ribozyme assays, RT-PCR, and lariat debranching assays to confirm RNA structures.
  • Analysis of protein-RNA complexes.

Main Results:

  • Two prominent box C/D RNA species were identified in P. furiosus, differing in electrophoretic mobility.

Related Experiment Videos

  • Evidence confirmed these species exist as both circular and linear RNAs of similar length and abundance.
  • Most, if not all, box C/D RNAs in P. furiosus appear to exist in a circular form.
  • Circular box C/D RNAs were found associated with functional proteins.

    • Conclusions:

    • Box C/D RNAs exist in a rare circular form in the hyperthermophilic archaeon Pyrococcus furiosus.
    • This discovery adds to the limited known examples of natural circular RNAs.
    • The findings suggest a previously unrecognized pathway for box C/D RNA biogenesis in archaea.