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Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
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Characterization of human anti-hrB-like monoclonal antibody

A P Blancher1, M E Reid, S J Alié-Daram

  • 1Laboratoire d'Immunologie, Pavillion Charles Lefebvre, CHU Purpan, 31059, Toulouse Cedex, France.

Immunohematology
|January 1, 1996
PubMed

To develop a blood typing reagent with Rh specificity, peripheral blood lymphocytes were isolated from a multiparous woman with apparent alloanti-Ce and fused with FIS heteromyeloma cells. One hybridoma cell line, FOR-2E3, secreted human IgG, which agglutinated all human red blood cells except R2R2, RZRZ, Dc-, D--, Rhnull, and e+ hrB-. The supernatant is useful as a reagent to screen for hrB- blood donors and to differentiate haplotypes with C and e in cis from haplotypes with C and e in trans.

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