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Human melanotransferrin (p97) has only one functional iron-binding site
E N Baker1, H M Baker, C A Smith
1Department of Chemistry and Biochemistry, Massey University, Palmerston North, New Zealand.
FEBS Letters
|February 24, 1992
Abstract:
The iron-binding properties of melanotransferrin, the tumour-associated antigen also known as p97, have been investigated by UV/visible and fluorescence spectroscopy, amino acid sequence comparison, and modelling. These show that, in contrast to other transferrins, melanotransferrin binds only one Fe3+ ion per molecule. The binding properties of its N-terminal site are similar to other transferrins, but its C-terminal site does not bind iron at all. The differences can be related to specific amino acid changes in the C-terminal site.