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Updated: Aug 21, 2026

Anti-Nuclear Antibody Screening Using HEp-2 Cells
Published on: June 23, 2014
LISS-dependent autoantibody with apparent anti-U specificity
J T Chiofolo1, M E Reid, D Charles-Pierre
1New York Blood Center, Immunohematology Laboratory, 310 East 67th Street, New York, NY 10021, USA.
Abstract:
Nonspecific binding of gamma globulin and complement, resulting in falsely positive indirect antiglobulin tests, has occurred with use of low-ionic strength saline solution (LISS) reagents. These LISS-dependent antibodies have not been described in association with any particular disease state, nor do they have any apparent clinical significance. We present a LISS-dependent antibody with apparent autoanti-U specificity.
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