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LISS-dependent autoantibody with apparent anti-U specificity.
J T Chiofolo1, M E Reid, D Charles-Pierre
1New York Blood Center, Immunohematology Laboratory, 310 East 67th Street, New York, NY 10021, USA.
Immunohematology
|January 1, 1995
Summary
Low-ionic strength saline solution (LISS) can cause false positives in indirect antiglobulin tests due to antibody binding. This study identifies a specific LISS-dependent antibody with autoanti-U specificity.
Area of Science:
- Immunology
- Transfusion Medicine
Background:
- Low-ionic strength saline solution (LISS) reagents are used in blood banking to enhance antibody detection.
- Nonspecific binding of gamma globulin and complement to red blood cells can occur with LISS, leading to false-positive indirect antiglobulin tests (IAT).
- These LISS-dependent antibodies have not been previously associated with specific disease states or clinical significance.
Purpose of the Study:
- To describe a novel case of a LISS-dependent antibody.
- To investigate the specificity of this LISS-dependent antibody.
Main Methods:
- Indirect antiglobulin tests were performed using LISS reagents.
- Antibody specificity was determined through antibody identification panels.
- The antibody was characterized for its reactivity and dependence on LISS.
Main Results:
- A LISS-dependent antibody was identified.
- This antibody demonstrated specificity for the U antigen (autoanti-U).
- The antibody caused false-positive results in IAT when LISS was used.
Conclusions:
- LISS reagents can induce or enhance the detection of specific antibodies, including autoanti-U.
- The identification of this autoanti-U antibody highlights the importance of understanding LISS-dependent phenomena in transfusion medicine.
- Further investigation is warranted to determine the clinical significance, if any, of LISS-dependent autoanti-U antibodies.