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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

795
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
795
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

596
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
596
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

4.0K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
4.0K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

1.1K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.1K
Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

1.6K
The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
1.6K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

7.3K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Related Experiment Video

Updated: Oct 23, 2025

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
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Raman spectroscopy in chemical bioanalysis.

Josefa R Baena1, Bernhard Lendl

  • 1Analytical Chemistry Department, Annex C-3 building, Campus de Rabanales, University of Córdoba, 14071- Córdoba, Spain.

Current Opinion in Chemical Biology
|September 29, 2004
PubMed
Summary

Raman spectroscopy, a powerful technique, is increasingly used in biochemical applications due to its sensitivity and non-invasive nature. Recent advancements enhance its capabilities for diverse fields like clinical pathology and microorganism detection.

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Area of Science:

  • Biochemistry
  • Spectroscopy

Background:

  • Raman spectroscopy offers sensitivity to structural changes, non-invasive sampling, and high spatial resolution.
  • Instrumentation advances are expanding its use in biochemical applications.

Purpose of the Study:

  • To review recent technical advancements in Raman spectroscopy.
  • To highlight key applications in various scientific and medical fields.

Main Methods:

  • Discussion of surface-enhanced resonance Raman spectroscopy (SERRS).
  • Exploration of non-linear Raman techniques like coherent anti-Stokes Raman spectroscopy (CARS).

Main Results:

  • Raman spectroscopy is becoming a preferred tool for many (bio)chemical analyses.
  • Recent applications span clinical pathology, in vivo/ex vivo imaging, microorganism detection, and chemical analysis.

Conclusions:

  • Raman spectroscopy's versatility and recent technical progress support its growing importance.
  • This technique holds significant promise for future advancements in chemical and biological sciences.