Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

RNA-directed DNA methylation.

Olivier Mathieu1, Judith Bender

  • 1Department of Biochemistry and Molecular Biology, Johns Hopkins University Bloomberg School of Public Health, 615 N. Wolfe Street, Baltimore, MD 21205, USA.

Journal of Cell Science
|October 1, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Porcine xenotransplantation in the clinical era: converging advances and unresolved barriers on the path to clinical translation - a narrative review.

Frontiers in immunology·2026
Same author

Highly dynamic metabolic response of grapevine to water deficits reveals an adaptability to a wide range of climatic conditions.

Food chemistry: X·2026
Same author

Patient-reported outcomes following chest masculinization surgery in transmasculine individuals: A prospective study.

JPRAS open·2026
Same author

Shaping the Future of Nasal Surgery: A Systematic Review of Preservation Rhinoplasty.

Aesthetic plastic surgery·2026
Same author

Uterus transplantation in the United States: analysis of patients and early postoperative outcomes in the national organ procurement and transplantation network.

Frontiers in transplantation·2026
Same author

Reflectance confocal microscopy in the management of lentigo maligna and lentigo maligna melanoma: a systematic review.

JPRAS open·2026
Same journal

Disentangling the response to lysosomal damage.

Journal of cell science·2026
Same journal

The force, form and function of the nucleus.

Journal of cell science·2026
Same journal

The nucleus-vacuole junction at a glance.

Journal of cell science·2026
Same journal

Loss of INPP5E affects photoreceptor outer segment membrane biogenesis in iPSC-derived human retinal organoids.

Journal of cell science·2026
Same journal

Brinker regulates reciprocal outcomes of BMP signal between stem cells and differentiating cells.

Journal of cell science·2026
Same journal

Primary cilium disassembly - from mechanisms to roles in physiology and disease.

Journal of cell science·2026
See all related articles

Small RNAs guide gene regulation by targeting messenger RNAs or DNA. In plants, double-stranded RNAs (dsRNAs) direct DNA methylation for heterochromatin formation, impacting gene accessibility.

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Genetics

Background:

  • Double-stranded RNAs (dsRNAs) and small RNAs regulate gene expression in eukaryotes.
  • RNA-directed mechanisms can degrade messenger RNAs post-transcriptionally.
  • dsRNA-derived species also influence chromatin structure and gene silencing.

Purpose of the Study:

  • To explore how RNA molecules can alter DNA accessibility and gene expression.
  • To understand the role of RNA in directing DNA methylation and heterochromatin formation.
  • To investigate the mechanisms linking RNA signals to DNA methylation imprints.

Main Methods:

  • Analysis of RNA-directed gene silencing pathways.
  • Investigating the role of dsRNAs in chromatin modification.

Related Experiment Videos

  • Studying DNA methylation processes in plants, fission yeast, and Drosophila.

    • Main Results:

    • Plants utilize dsRNA species to induce cytosine methylation on homologous DNA sequences.
    • This RNA-directed DNA methylation (RdDM) establishes transcriptionally silent heterochromatin.
    • RNA can modulate DNA accessibility, affecting gene expression.
    • Heterochromatin formation guided by RNA occurs independently of DNA methylation in yeast and Drosophila.

    Conclusions:

    • RNA plays a crucial role in epigenetic regulation beyond post-transcriptional gene silencing.
    • RNA-directed DNA methylation is a key mechanism for heterochromatin formation in plants.
    • The study highlights conserved and divergent mechanisms of RNA-guided genome regulation across eukaryotes.