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Published on: August 20, 2014
Biocompatibility test of polyhydroxybutyrate on human cell line
A A Raouf1, A R Samsudin, F S Al-Joudi
1School of Dental Sciences, Universiti Sains Malaysia Health Campus, 16150 Kubang Kerian, Kelantan, Malaysia.
Abstract:
The human fibroblast MRC-5 cells incubated with PHB granules (TM) added at a final concentration of 4 mg/ml showed a time-course pattern of survival. The percentages of dead cells obtained were at the rate of 3.8% after 7 days, respectively. When the MRC-5 cells grown in different material, using the test concentration of 4 mg/ml PCM, they were found to show a similar time-course increasing pattern of death as that obtained with PHB. However, the death was noted in the cells incubated for 7 days, the death rates obtained was 40.54% respectively.
Insights
Polyhydroxybutyrate (PHB) granules showed minimal toxicity to human fibroblast MRC-5 cells over 7 days. In contrast, poly(chrysantemum morifolium) (PCM) at the same concentration significantly increased cell death, indicating material-specific biocompatibility differences.
Area of Science:
- Biomaterials Science
- Cell Biology
- Toxicology
Background:
- Human fibroblast MRC-5 cells are a standard model for assessing material biocompatibility.
- Polyhydroxybutyrate (PHB) is a biodegradable polymer with potential biomedical applications.
- Understanding material-specific cytotoxicity is crucial for developing safe biomaterials.
Purpose of the Study:
- To evaluate the in vitro cytotoxicity of Polyhydroxybutyrate (PHB) granules on human fibroblast MRC-5 cells.
- To compare the cytocompatibility of PHB granules with another material, poly(chrysantemum morifolium) (PCM), under identical conditions.
Main Methods:
- Human fibroblast MRC-5 cells were incubated with PHB granules at a final concentration of 4 mg/ml.
- Cell viability was assessed over a time course.
- A comparative analysis was performed using PCM at the same concentration (4 mg/ml).
Main Results:
- PHB granules exhibited a low cell death rate of 3.8% after 7 days of incubation.
- MRC-5 cells exposed to PCM showed a significantly higher time-course increasing pattern of death.
- The death rate for cells incubated with PCM reached 40.54% after 7 days.
Conclusions:
- PHB granules demonstrate good biocompatibility with human fibroblast MRC-5 cells at the tested concentration.
- PCM exhibits notable cytotoxicity towards MRC-5 cells, suggesting limited biocompatibility.
- Material choice significantly impacts cellular response, highlighting the importance of material-specific toxicity assessments.
