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Determination of d-alpha-tocopherol and its impurity by chiral high performance liquid chromatography in

Gabriela Kłaczkow1, Dorota Jakoniuk, Elzbieta L Anuszewska

  • 1National Institute of Public Health, Department of Biochemistry and Biopharmaceuticals 30/34 Chełmska Str., 00-725 Warsaw, Poland.

Acta Poloniae Pharmaceutica
|October 16, 2004
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High-Performance Liquid Chromatography: Introduction01:11

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process: