[Assessment of liver graft function after cold preservation using 31P and 23Na NMR spectroscopy]

We investigated the functional damages of cytoplasm and cell membrane of liver grafts in male WKY rats after 24hr- or 48hr-cold preservation using a UW solution in vitro. 10mM fructose or 50mM NH4Cl was added to the perfusate, and synthesis of fructose-1-phosphate (F-1-P) and Na(+)- and H(+)-ion transports through the cell membrane were evaluated with 31P-MRS and 23Na-MRS. After 30 minutes of reperfusion, beta-ATP/(inorganic phosphate: Pi) of the 48hr-preserved liver was significantly lower than those of the other groups. In the control and the 24hr-preserved liver, the changes of F-1-P were almost the same, but F-1-P synthesis was lower in the 48hr-preserved liver than those of the other groups. Intracellular pH began to drop after cessation of NH4Cl loading, and then recovered to the previous level. At the same time Nain was increased in the control group. However, in the other two groups, the increasing rates of Nain were lower, and the recoveries of Nain were worse. In conclusion, the function of cell membrane was more fragile than that of mitochondria and cytoplasmic sugar metabolism in the liver graft.