Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[mtDNA mutations in mouse tumors].

Ji-gang Dai1, Jia-xin Min, Guo-qiang Zhang

  • 1Department of Thoracic Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.

Zhonghua Bing Li Xue Za Zhi = Chinese Journal of Pathology
|October 23, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural features of endogenous polyphenols in modulating oxidative stability of tiger nut (Cyperus esculentus L.) oil: Insights from Rancimat and density functional theory.

Food chemistry·2026
Same author

Delayed Arousal Response to Sleep Apnea Encodes Mortality.

medRxiv : the preprint server for health sciences·2026
Same author

SERPINE1 drives ferroptosis in acute respiratory distress syndrome by disrupting mitochondrial NAD<sup>+</sup> homeostasis and suppressing Sirt3 activity.

Redox biology·2026
Same author

Protocol for detecting genome-wide introgressed genes and evaluating their functional legacy.

STAR protocols·2026
Same author

A layered standards framework for integrating single-cell and spatial omics data into brain cell atlases.

bioRxiv : the preprint server for biology·2026
Same author

A Community Standard Multispecies Cell Atlas of the Basal Ganglia.

bioRxiv : the preprint server for biology·2026

Mitochondrial DNA (mtDNA) mutations in the D-loop region were identified in mouse tumors. These D-loop mutations may play a role in the development of murine cancers.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Mitochondrial DNA (mtDNA) plays a crucial role in cellular energy production.
  • Alterations in mtDNA have been implicated in various diseases, including cancer.
  • Understanding mtDNA variations in tumors is essential for elucidating carcinogenesis mechanisms.

Purpose of the Study:

  • To investigate variations in mitochondrial DNA (mtDNA) within mouse tumors.
  • To explore the potential relationship between mtDNA mutations and the development of murine cancers.

Main Methods:

  • Analysis of D-loop, ND3, and tRNAIle + Glu + Met gene fragments of mtDNA from six mouse tumor cell lines.
  • Utilized Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) techniques.

Related Experiment Videos

Main Results:

  • No variations were observed in ND3 and tRNAIle + Glu + Met gene fragments at 27 endonuclease sites.
  • The D-loop of mtDNA in the Hca-F tumor line exhibited an additional Hinf I endonuclease site compared to inbred mice.
  • PCR-SSCP analysis revealed mutations in the D-loop of mtDNA in 4 out of 6 analyzed tumors.

Conclusions:

  • The D-loop region of mtDNA appears to be a 'hot spot' for mutations in tumors.
  • These mtDNA mutations in the D-loop may contribute to the process of carcinogenesis in murine tumors.