Related Experiment Video
Updated: May 21, 2026

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library
Published on: January 10, 2025
Protocol for detecting genome-wide introgressed genes and evaluating their functional legacy
Zhenbin Jiao1, Zhiyao Ren2, Chao Hu3
1Key Laboratory of Orchid Conservation and Utilization of National Forestry and Grassland Administration at College of Landscape Architecture and Art, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Abstract:
Introgressed genes can significantly influence the ecological fate of recipient species exposed to novel environments. Here, we present a protocol for detecting introgressed genes and assessing their functional impact in recipient species by combining fdM statistics with expression profiling under various stress conditions. We describe procedures for identifying introgressed genes, their paralogs, and their alleles. We also detail steps for analyzing and comparing their responses to abiotic stress in orchids. For complete details on the use and execution of this protocol, please refer to Jiao et al.1.
Related Concept Videos
Genomics
DNA-only Transposons
The donor site from where the transposon is excised is either degraded or...
Genetic Screens
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...
Genome-wide Association Studies-GWAS
GWAS does not require the identification of the target gene involved in...
Genomic DNA in Eukaryotes

