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Protocol for detecting genome-wide introgressed genes and evaluating their functional legacy.

Zhenbin Jiao¹, Zhiyao Ren², Chao Hu³

¹Key Laboratory of Orchid Conservation and Utilization of National Forestry and Grassland Administration at College of Landscape Architecture and Art, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; University of Chinese Academy of Sciences, Beijing 100049, China.

|May 19, 2026

Summary

This study introduces a new protocol to detect introgressed genes and analyze their functional impact in recipient species. The method combines population genetics statistics with gene expression analysis to understand adaptation to new environments.

Keywords:

Evolutionary biology Genetics Plant sciences

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Area of Science:

Evolutionary Biology
Genomics
Plant Science

Background:

Introgressed genes can facilitate adaptation to novel environments.
Understanding the functional role of these genes is crucial for recipient species' ecological success.

Purpose of the Study:

To present a protocol for detecting introgressed genes and assessing their functional impact.
To analyze the response of introgressed genes to abiotic stress in orchids.

Main Methods:

Combining f_dM statistics with gene expression profiling.
Identifying introgressed genes, their paralogs, and alleles.
Analyzing gene responses to abiotic stress conditions.

Main Results:

The protocol enables the identification and functional assessment of introgressed genes.
Differential gene expression analysis reveals responses to stress.
This approach aids in understanding adaptive introgression.

Conclusions:

The presented protocol is effective for studying introgressed genes and their adaptive roles.
This method can be applied to various species facing environmental changes.
It provides insights into the genetic basis of ecological adaptation.