Related Experiment Video
Updated: Aug 21, 2026
Endoscopic Cholesteatoma Surgery
Published on: January 19, 2022
[The expression of matrix metalloproteinase-9 and tumor necrosis factor-alpha in cholesteatoma]
Nan Li1, Zhaobing Qin, Mingmin Dong
1Department of Otolaryngology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
Objective:
To observe the expression of MMP-9, TNF-alpha in cholesteatoma and to determine their roles in the destruction of bone.
Method:
Immunohistochemical method (SP) and the computer image quantitative analysis were used to examine the expression of TNF-alpha, MMP-9 in 22 samples from cholesteatoma and 20 normal cases. At the same time, the correlation between the expressions of the two indexes and the erosion of ossicles were observed.
Result:
MMP-9 protein was only expressed in cholesteatoma stromal cells. TNF-alpha was expressed in both epithlium and stromal cells. The results of the computer image quantitative analysis showed that the mean optical density of MMP-9 and TNF-alpha in cholesteatoma were higher than that in normal skin epithelial tissue (P < 0.05), in addition, the two indexes were relative to the extent of ossicle destruction.
Conclusion:
MMP-9 and TNF-alpha are overexpressed in cholesteatoma and they are associated with ossicle destruction, which shows that MMP-9 and TNF-alpha are responsible for bone destruction in cholesteatoma; MMP-9 and TNF-alpha have a correlation in their expression, which suggest that the two factors act on each other possibly; Overexpression of MMP-9 is only observed in stromal cells and the immunostain of TNF-alpha also is observed in stromal cells which indicates that stromal cells play an important role in bone destruction.
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