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Updated: Aug 21, 2026

Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry
Published on: December 13, 2013
[The study of immunofluorescence on the retinal digest preparations]
Jianxian Lin1, Ningyan Bai, Shibo Tang
1Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
Purpose:
To study the technique of immunofluorescence staining on the digested retina preparations.
Methods:
Retinas of 5 normal Wistar rats and 5 STZ diabetic Wistar rats of were used to make digested retina preparations and carry out immunofluorescence staining with the vascular cell adhesion molecule-1 (VCAM-1).
Result:
The retinal vessels were clearly observed; There were a lot of positive cells in the retinal preparations of the diabetic Wistar rats, while there were no positive cells in the retinal preparations of the normal rats.
Conclusion:
It is feasible to carry out immunofluorescence staining on the digested retina preparations.
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